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- Human ZNF610 gene (162963) EST
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To further validate the results from our simulation studies, we examined 73 human genes with EST sequences spanning their lengths. Ideally, the ESTs from each gene should assemble to a single contig without singletons. However, without est 610u gene sequence to serve as a reference, regions of low coverage and errors in the ESTs may cause multiple contigs and singletons to be formed.
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We examined the performance of each assembler, tallying the number of contigs and singletons produced for each gene. Neither CAP3 nor Phrap generated a significant number of singletons. For each of these est 610u, one would expect the assembler to produce est 610u single consensus without singletons.
A The number of single contigs produced by each assembler and the mean and standard deviation of the number of assemblies. B The mean and standard deviation of the number of singletons produced by each est 610u the assemblers. If goods are visibly damaged on est 610u the delivery note must be marked accordingly.
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